NcoI-HF R3193S from New England Biolabs

Product Specs
ItemNcoI-HF
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR3193S
Concentration20,000 units/ml
Quantity1000 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

C⇓CATGG
GGTAC⇑C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Activation of Csm6 ribonuclease by cyclic nucleotide binding: in an emergency, twist to openNucleic Acids ResearchOctober 27, 2023Stuart McQuarrie, Januka S Athukoralage, Stephen A McMahon, Shirley Graham, Katrin Ackermann, Bela E Bode, Malcolm F White, Tracey M GlosterInc.). Reagents NcoI and BamHI restriction enzymes (New England Biolabs, Hitchin, Herts, UK, cat. no. R3193S and R0136S respectively); QuikChange Site-Directed Mutagenesis kit (Agilent Technologies, Santa Clara,

(2) An efficient cloning method to expand vector and restriction site compatibility of Golden Gate AssemblyCell Reports MethodsAugust 22, 2023Masato Sorida, Roberto Bonasio,Thermo Scientific,SM1331 BsaI-HFv2,NEB,R3733S BbsI-HF,NEB,R3539S AgeI-HF,NEB,R3552S BamHI-HF,NEB,R3136S EcoRI-HF,NEB,R3101L HindIII-HF,NEB,R3104S NcoI-HF,NEB,R3193S NotI-HF,NEB,R3189S SalI-HF,NEB,R3138S SpeI-HF,NEB,R3133S XbaI,NEB,R0145S XhoI,NEB,R0146S XmaI,NEB,R0180S KpnI-HF,NEB,R3142S NdeI,NEB,R0111S Smal,NEB,R0141S BbsI-HF,NEB,R3539M T4 DNA Ligase

(3) Large-scale Purification of Type III Toxin-antitoxin Ribonucleoprotein Complex and its Components from Escherichia coli for Biophysical StudiesBIO-PROTOCOLJuly 5, 2023Parthasarathy Manikandan, Kavyashree Nadig, Mahavir SinghLaboratories, catalog number: 41721, CAS number: 7647-14-5)NcoI (New England Biolabs, catalog number: R3193S), store at -20 °CNdeI (New England Biolabs, catalog number: R0111S), store at -20 °CPCR purification

(4) The conserved histone chaperone Spt6 is strongly required for DNA replication and genome stabilityCell ReportsMarch 28, 2023Catherine L.W. Miller, Fred Winstonverticillus,Sigma,P9564 3-Indoleacetic acid (IAA),Sigma,12886 RnaseH,NEB,M0297L AccI,NEB,R0161S EcoRV,NEB,R3195S NcoI,NEB,R3193S HaeIII,NEB,R0108S BsrG1,NEB,R3575S SigmaFAST protease inhibitor cocktail tablets,Sigma,S8820-20TAB RNaseA/T1

(5) Protocol for investigating the biogenesis of SARS-CoV-2 S pseudoviruses in HEK293T cells transduced to express the virus-specific intrabodiesSTAR ProtocolsMarch 1, 2023Surbhi Dahiya, Sudhakar Singh, Sharvan SehrawatFemtoLUCENT plus AP kit,G-Biosciences,Cat#786-10AP PEI branched,Sigma,408727-100mL BamHI,New England Biolab (NEB),R3136S XbaI,NEB,R0145S NcoI,NEB,R3193S NotI,NEB,R3189S MG132,Sigma,474787 Ni-NTA probes,ForteBio,18-5101 Clarity Western ECL substrate,Bio-Rad,Cat#170-5060 pNPP

(6) Regulation of hierarchical carbon substrate utilization, nitrogen fixation, and root colonization by the Hfq/Crc/CrcZY genes in Pseudomonas stutzeriiScienceDecember 22, 2022Fanyang Lv, Yuhua Zhan, Wei Lu, Xiubin Ke, Yahui Shao, Yiyuan Ma, Juan Zheng, Zhimin Yang, Shanshan Jiang, Liguo Shang, et al.III-HF,NEB,Cat# R3104S Nde I,NEB,Cat# R0111S Spe I-HF,NEB,Cat# R3133S Xho I,NEB,Cat# R0146S Nco I-HF,NEB,Cat# R3193S 4-Methylumbelliferyl-b-D-galactoside,Sigma-Aldrich,Cat# 4368814 7-Hydroxy-4-methylcoumarin,Sigma-Aldrich,Cat#

(7) Glucosamine amends CNS pathology in mucopolysaccharidosis IIIC mouse expressing misfolded HGSNATThe Journal of experimental medicineAugust 1, 2022Pan X, Taherzadeh M, Bose P, Heon-Roberts R, Nguyen ALA, Xu T, Pará C, Yamanaka Y, Priestman DA, Platt FM, et al.distinguish between mutant and WT amplicons, the PCR products were further digested with NcoI (Cat# R3193S; New England Biolabs) at 37°C for 1 h and then run on a 2% agarose gel. Fragments of 688 and 300 bp

(8) Robust anti-SARS-CoV2 single domain antibodies cross neutralize multiple virusesiScienceJuly 15, 2022Sudhakar Singh, Surbhi Dahiya, Yuviana J. Singh, Komal Beeton, Ayush Jain, Roman Sarkar, Abhishek Dubey, Azeez Tehseen, Sharvan Sehrawat,G Biosciences,Cat#786-10AP PEI Branched,Sigma,408727-100mL BamHI,NEB,R3136S XbaI,NEB,R0145S NcoI,NEB,R3193S NotI,NEB,R3189S MG132,Sigma,474787 Ni-NTA probes,ForteBio,18-5101 Clarity Western ECL substrate,Bio-Rad,Cat#170-5060 pNPP

(9) Orally active bivalent VHH construct prevents proliferation of F4+ enterotoxigenic Escherichia coli in weaned pigletsiScienceApril 15, 2022Berthe Katrine Fiil et al.proteins","Chemicals, peptides, and recombinant proteins" ,, NcoI restriction enzyme,New England Biolabs,R3193S NotI restriction enzyme,New England Biolabs,R3189S T4 DNA ligase,New England Biolabs,M0202S Benzonase

(10) TranSNPs: A class of functional SNPs affecting mRNA translation potential revealed by fraction-based allelic imbalanceiScienceDecember 17, 2021Samuel Valentini, Caterina Marchioretti, Alessandra Bisio, Annalisa Rossi, Sara Zaccara, Alessandro Romanel, Alberto Ingachemicals,Cat#10004372 Doxorubicin,Sigma Aldrich,Cat#25316-40-9 Xba I,New England Biolabs,Cat# R0145S Nco I,New England Biolabs,Cat# R3193S T4 DNA ligase,New England Biolabs,Cat# M0202S Protein A Magnetic Beads,Thermo Fisher Scientific,Cat#

(11) Multiplexed characterization of rationally designed promoter architectures deconstructs combinatorial logic for IPTG-inducible systemsNature CommunicationsDecember 1, 2021Timothy C. Yu et al.99 and GU 100 at 10 µM. This amplicon was then digested with BsaI-HF (NEB # R3535) and NcoI-HF (NEB #R3193S) for 1.5 h at 37 °C. pLib was digested with BsaI-HF (NEB # R3535) and NheI (NEB# R3131S). pLib vector

(12) Fetuin-A is a HIF target that safeguards tissue integrity during hypoxic stressNature CommunicationsDecember 1, 2021Stefan Rudloff, Mathilde Janot, Stephane Rodriguez, Kevin Dessalle, Willi Jahnen-Dechent, Uyen Huynh-DopGL3-basic-P2P-607 plasmid (kind gift of Prof. Wenger, Zurich) with NcoI and SacI restriction enzymes (both NEB, R3193S, and R3156S). Intronic fragments were inserted into the pGL3-basic vector or the pGL3-basic vector carrying

(13) Monoclonal Human Antibodies That Recognise the Exposed N and C Terminal Regions of the Often-Overlooked SARS-CoV-2 ORF3a Transmembrane ProteinVirusesNovember 2, 2021Tan TH, Patton E, Munro CA, Corzo-Leon DE, Porter AJ, Palliyil S.single chain Fv (scFv) genes isolated by restriction digestion using NotI-HF and NcoI-HF (R3189S and R3193S, New England Biolabs, Ipswich, MA, USA) and cloned into the bacterial expression vector pIMS147 [41].

(14) A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoirCell Reports MedicineApril 20, 2021Claire N. Levy et al.Santa Cruz Biotechnology Inc.,Cat#SC-202638CAS No. 593-84-0 KpnI-HF,New England BioLabs,Cat#R3142S NcoI-HF,New England BioLabs,Cat#R3193S NsiI-HF,New England BioLabs,Cat#R3127S PacI,New England BioLabs,Cat#R0547S SalI-HF,New England BioLabs,Cat#R3138S SbfI-HF,New England BioLabs

(15) THE ANALYSIS OF THE ASSOCIATION OF ADRB2 GENE POLYMORPHISM AND EXTERNAL RESPIRATORY FUNCTION IN CHILDREN WITH BRONCHIAL ASTHMAVestnik of Vitebsk State Medical UniversityApril 15, 2021E.S. Minina et al.секунд. Конечный этап +72°С – 5 минут. Рестрикцию проводили с использованием фермента NcoI-HF® (NEB, США) кат. № R3193S с CutSmart буферным раствором, согласно протоколу производителя. Для проведения 1 реакции использовали

(16) High-resolution structure of eukaryotic Fibrillarin interacting with Nop56 amino-terminal domainRNAApril 1, 2021Simone Höfler, Peer Lukat, Wulf Blankenfeldt, Teresa CarlomagnoM0530S). All forward and reverse primers contained the cleavage site for NcoI-HF (New England Biolabs R3193S) and NotI-HF (New England Biolabs R3189S), respectively. Amplified genes were purified using the QIAquick

(17) Tissue-Specific In Vivo Biotin Chromatin Immunoprecipitation with Sequencing in Zebrafish and ChickenSTAR ProtocolsOctober 28, 2020Martyna Lukoseviciute, Irving T.C. Ling, Upeka Senanayake, Ivan Candido-Ferreira, Gunes Taylor, Ruth M. Williams, Tatjana Sauka-SpenglerDodecyl Sulfate (SDS) powder,Sigma-Aldrich,Cat# L4509CAS: 151-21-3 High Fidelity (HF) NcoI,NEB,Cat#R3193S SnaBI Restriction endonuclease,NEB,Cat#R0130 High Fidelity (HF) EcoRV,NEB,Cat#R3195 "Proteinase K, recombinant, PCR Grade

(18) Simultaneous Examination of Cellular Pathways using Multiplex Hextuple Luciferase AssayingCurrent Protocols in Molecular BiologyJune 26, 2020Alejandro Sarrion‐Perdigones, Lyra Chang, Yezabel Gonzalez, Tatiana Gallego‐Flores, Damian W. Young, Koen J.T. Venkenno. R0580S), MfeI-HF (New England Biolabs, cat. no. R3589S), NcoI-HF (New England Biolabs, cat. no. R3193S), and XhoI (New England Biolabs, cat. no. R0146S). Restriction enzyme buffer: CutSmart® buffer (New England Biolabs

(19) Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotectionNature communicationsFebruary 18, 2020Wei Y, Lu M, Mei M, Wang H, Han Z, Chen M, Yao H, Song N, Ding X, Ding J, et al.amplification products were divided into four aliquots for digestion with NcoI (New England Biolabs, NEB-R3193S), PstI (New England Biolabs, NEB-R3140S), both enzymes, or neither enzyme at 37 °C for 30 min. The digestion

(20) Two Faces of CwlM, an Essential PknB Substrate, in Mycobacterium tuberculosisCell reportsOctober 2, 2018Turapov O, Forti F, Kadhim B, Ghisotti D, Sassine J, Straatman-Iwanowska A, Bottrill AR, Moynihan PJ, Wallis R, Barthe P, et al.Platinum Taq DNA polymerase,ThermoFisher Scientific,Cat# 10966034 Restriction enzymes,New England Biolabs(UK) Ltd- /,Cat #R3193S; R3182L; R0111L; R3136L; R3142L LigaFast Rapid DNA Ligation System,Promega,Cat# M8221 SignalFire Elite ECL Reagent

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4/30/2018Diagnostic Restriction Mapping Of The Plasmid Construct With NcoI-HF Triveni MahendrappaI have constructed a plasmid construct called pCU190_dgp of a size approximately 19 kb by Gibson assembly method. This plasmid prepped from carrier E.coli cells was subjected to diagnostic restriction... Read Review

NcoI-HF

NcoI-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (25)

Reviews

NcoI-HF from New England Biolabs

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